Kavitha K*, Muthu Mohamed J and Chitra Karthikeyini S
Background: This study investigates the inclusion complex of curcumin (CMN) enhance the solubility, which can be utilized for the treatment of colorectal cancer (CRC) greater to free CMN.
Methods:CMN solid dispersion (SD) prepared by a hot melt method using CMN with several carriers of poloxamers (P-407 and P-188), gelucire 50/13 (GLR) and mannitol (MNT). Prior, molecular modelling and phase solubility studieswere performed with drug and carriers. The SD characterized by in vitrodrug release, SEM and functionalize dyeing test. Additionally, the cytotoxicity and image of apoptosisresolved to utilize the colorectal adenocarcinoma cell lines.
Results: The result showed that CMN-P-407 inclusion complex produced significant properties towards solubility (318 ± 14.46 fold) and dissolution (91 ± 0.431% at 30 min). Similarly, these data fit with insilico model. The IC50 value for inclusion complex found to be 74 and 52 µM/mL, while that for free CMN ranged from 146 and 116 µM/mL on the SW480 and Caco-2 cells respectively. Apoptosis study described that the cells are undergoing cell death by apoptosis and the small number of necrosis.
Conclusion: The profound efﬁciency of CMN-P-407 SD indicated its potential application for CRC treatment by showing a higher capability of inhibiting cell growth compared to that of free CMN.
Curcumin, Functionalized dyeing test, Phase solubility studies, Colorectal cancer, MTT assay, Hoechst staining.
Department of Pharmaceutical Technology, BIT campus, Anna University, Tiruchirappalli 620024, Tamil Nadu, , Department of Pharmaceutical Technology, BIT campus, Anna University, Tiruchirappalli 620024, Tamil Nadu, , Department of Pharmaceutical Technology, BIT campus, Anna University, Tiruchirappalli 620024, Tamil Nadu